RNA

Part:BBa_K3425039:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-17)


Qβ3' recognition sequence of Qβ replicase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled further using the Type IIS iGEM standard assembly. You can find more information about the cloning of this part as well as similar parts, which do not fit into the four predefined categories in Type IIS standard (promoter, RBS, CDS, terminator) in the Type IIS guidebookof Uppsala 2020 team.

Source

Synthesized according to
[1] Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

References

[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078