Part:BBa_K3425039:Design
Qβ3' recognition sequence of Qβ replicase
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 15
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 46
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was assembled further using the Type IIS iGEM standard assembly. You can find more information about the cloning of this part as well as similar parts, which do not fit into the four predefined categories in Type IIS standard (promoter, RBS, CDS, terminator) in the Type IIS guidebookof Uppsala 2020 team.
Source
Synthesized according to
[1] Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.
References
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078